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Research Article | Open Access
Volume 3 | Issue 3 | Year 2016 | Article Id. IJAP-V3I6P104 | DOI : https://doi.org/10.14445/23500301/IJAP-V3I6P104

Radio Frequency Stimulus Constrains the Tryptophan Synthase Beta Subunit

Dong Fu, Heng han

Citation :

Dong Fu, Heng han, "Radio Frequency Stimulus Constrains the Tryptophan Synthase Beta Subunit," International Journal of Applied Physics, vol. 3, no. 3, pp. 24-28, 2016. Crossref, https://doi.org/10.14445/23500301/IJAP-V3I6P104

Abstract

To prove the capability of the Nativis signal transduction technology to moderate the appearance of algae mRNA and protein, we verified if can change exact enzyme stages in Chlamydomonas reinhardtii. We reserved the mixture of the enzyme tryptophan synthase beta subunit by applying the signal derivative from a available siRNA. With inferior levels of MAA7, Chlamydomonas reinhardtii can produce in the attendance of the prodrug 5-Fluoroindole (5-FI), because less 5-Fluoroindole can be altered to the toxic 5-Fluoro-L-tryptophan. We find a 24% increase of growth with the indication versus no signal. To see if that result was due to the decrease of the quantity of mRNA encoding MAA7, we used Real-Time Quantitative PCR (RT-QPCR) to amount the stages of MAA7 mRNA. To regularize the MAA7 mRNA level, we likened them to the levels of a mRNA that is not affected by the signal (G protein beta subunit-like polypeptide, Cblp). Two situations upsurge the efficiency of the signal. One can also treat the cell cultures through the logarithmic growth stage. One can treat the cultures at a advanced period of the logarithmic development, but treating them for a longer time. Below these situations we found about a 50% reduction in the mRNA levels for MAA7. Treating the cultures at the earlier growth phase or at a later growth phase is less effective, with only a 20% effect.

Keywords

Ultra Low Radio Frequency Energy, ulRFE, siRNA, Oil Production, Nativis Technology, Beta Subunit of Tryptophan Synthase, Chlamydomonas reinhardtii

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