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Research Article | Open Access
Volume 6 | Issue 1 | Year 2019 | Article Id. IJAES-V6I1P104 | DOI : https://doi.org/10.14445/23942568/IJAES-V6I1P104

In Vitro Antioxidant Potentials and total Phenolic Content of Tunisian Datura Metel Extracts from Leaves and Seeds

Leyla Tahrani, Fathia Harzallah-Skhiri, Mahjoub Aouni and Karima Bel Hadj Salah

Citation :

Leyla Tahrani, Fathia Harzallah-Skhiri, Mahjoub Aouni and Karima Bel Hadj Salah, "In Vitro Antioxidant Potentials and total Phenolic Content of Tunisian Datura Metel Extracts from Leaves and Seeds," International Journal of Agriculture & Environmental Science, vol. 6, no. 1, pp. 24-28, 2019. Crossref, https://doi.org/10.14445/23942568/IJAES-V6I1P104

Abstract

The objective of this study is to determine the antioxidant activity and total phenols of Datura metel, a medicinal plant collected in Tunisia. Leaves and seeds of the plant were subject to cold maceration with solvent extracts: petroleum ether (PE), dichloromethane (CH2Cl2), ethyl acetate (ACOEt) and methanol (MeOH). Total phenolic contents were investigated by the method of Folin-Ciocalteu. Antioxidant activities of different extracts were determined by DPPH radical scavenging, ABTS assay and reducing power. All tested D. metel extracts showed interesting antioxidant activities and a difference was observed between leaves and seeds antioxidant potential extracts. Our results showed that the methanolic seeds extracts exhibited the highest total phenolic content. At 1 mg/ml concentration, the DPPH radical scavenging activities of leaves and seeds were 66.4% and 63.3% respectively, and the inhibition percentages of ABTS radical were between 96.54% and 97.01%. Our results suggest that D. metel extracts from both leaves and seeds could be used as potential sources of new antioxidant agents, useful in pharmacological and food industries.

Keywords

Datura metel, antioxidant activity, total phenolic content, seeds, leaves.

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